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BLUE - AGAROSE

References:

Kidney International (2003) 64, 748755; doi:10.1046/j.1523-1755.2003.00134.

CHARACTERISTICS OF THE GEL

Matrix: SepharoseTM CL-4B.
Activation method: Oxiran.
Ligand: Cibacron Blue®.
Binding capacity: Approx. 20 mg of HSA per ml of wet gel.
Packing: Suspension in PBS 50mM pH 7.4; NaN3 0.02% (w/v).
Stability of the gel: pH 2-11.
Life time of the gel with routine use: more than 10 cycles without significant loss of binding capacity.
Shelf life of the gel: several years if stored at 4C.
Storage: 4C.

ORDERING INFORMATION

Product code Product name Package *Price in EUR
BLU48 Blue-Agarose 4%
cross-linked
100 ml 260.00
500 ml 1200.00
1000 ml 2100.00
* All prices are FOB Liège.
Bulk quantities are available.

INSTRUCTION FOR USE (Albumin purification)

Gel volume: 100 ml Blue-Agarose.
Sample: 100 ml human serum.
Flow rate: 50 ml/cm2/hour for all operations.
Binding and washing buffer: PBS 50mM pH 8.0 (buffer A) or TBS 50mM pH 8.0 (buffer B).
Elution buffer: Buffer A or buffer B containing 1.5M NaCl.
Regeneration: With 5 vol. of distilled water then with 5 vol. of NaCl 2M and finally with 5 vol. of distilled water and 3 vol. of binding buffer.
Storage: 4C in the presence of NaN3 0.1% (w/v).

ABSORBANCE