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How to remove 'heavy metal ions' from your protein solution after IMAC elution steps?
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New!    How to remove "heavy metal ions"    New!
from your protein solution
after IMAC elution steps?

In IMAC purification using Ni-NTA, Co-NTA, Ni-Sepharose 6 fast flow, His-Link, Ni-TED..., the important concentration of imidazole used in elution steps will provoke a leakage of heavy metal ions from any metal chelate resin column...
Heavy metal ions are known toxic for cell growth as well as animal health.

References:

1) Environ. Toxicol. Chem. 2004 Jan; 23 (1) : 86-92.
2) J. Biol. Chem., Sep 1986; 261 : 14840 - 11848
3) J. Biol. Chem., Apr 2003; 278 : 12826 - 12833.
4) J. Biol. Chem., Sep 2004; 279 : 40337 - 40344.

How to remove "heavy metal ions"
from your protein solution
contaminated by these ions?

Itís easy, rapid and sure with
Affiland patented PMIB resin
as follows

  1. Add some PMIB (polyvalent metal ion binding resin) cake to your protein solution.
  2. Mix gently for 5 minutes.
  3. Stand 1-2 minutes and filter off the metal chelate PMIB from your protein solution which then becomes totally free of heavy metal ions.

For details on PMIB, see http://www.affiland.com/imac/pmib.htm.

In addition, to make sure the entire success in your IMAC purification,

Affiland can provide you with
"Complete PDC kit"
comprising

  1. 1 patented PDC kit for selecting the appropriate metal ion to be chelated, composed of
    1. 1 ml of Cu-PDCR resin column
    2. 1 ml of Zn-PDCR resin column
    3. 1 ml of Ni-PDCR resin column
    4. 1 ml of Co-PDCR resin column
    5. and
    6. 2 x 100 ml of Washing Buffer A (the chemical formula will be provided with the order)
    7. 2 x 100 ml of Washing Buffer B (the chemical formula will be provided with the order)
    8. 1 x 100 ml of Elution Buffer E (the chemical formula will be provided with the order)
    9. 1 x 100 ml of Elution Buffer F (the chemical formula will be provided with the order).
  2. 1 x 25 ml of PMIB for removing heavy metal ions from protein solutions eluted by imidazole containing Elution Buffers, without loss of protein content.
  3. 1 x 25 ml of PDC resin, free of metal ions for scaling up the purification of your his-tagged protein.

For details on PDC and PDC kit, see http://www.affiland.com/imac/pdc6x.htm.

If you are interested in
"how to remove imidazole from your protein solution without loss of protein content",
we will provide you with our special affinity gel for it