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Affiland

Activated PROTEIN A - SepharoseTM Fast Flow
for
IgG (mouse, rabbit, human...) IMMOBILIZATION KIT
(easy-to-use)
For rapid antigen purification by immunoaffinity column

It is not difficult to immobilize a protein on a solid support using any classic activation method such as the cyanogen bromide or N-hydroxysuccinimidylcarboxylate... However, if the protein of interest is a specific IgG, its biological activity will be affected if not entirely lost after the covalent linkage between the solid support with the terminal H2N of the IgG.

AFFILAND has developed the Activated Protein A-Sepharose fast flow useful for the immobilization of IgG on the Fc portion, leaving free the Fab: the biological properties of the specific IgG will be conserved.

CHARACTERISTICS OF THE GEL

Matrix: SepharoseTM 4 Fast Flow
Ligand: Activated Protein A which has a specific binding for the Fc of IgG. It does not recognize serum albumin or serum proteins other than immunoglobulins.
Rapid formation of a stable complex between the ligand and the Fc portion of immunoglobulin from IgG from mouse, rabbit, human..., in the binding & coupling buffer, followed by an immediate covalent linkage between the Activated matrix and the Fc portion, leaving free the Fab of IgG.
Easily realized in a column.
Sample: can be either the antisera, ascites fluid, extract containing antibodies or purified IgG.
Stability of the matrix: pH 6-9.
Storage: 4C in PBS 50mM pH 7.4; NaN3 0.02% (w/v).

Results

Easy recovery of the antibody immobilized on the gel in a record time of 30 minutes maximum. The capacity of the obtained affinity matrix may be 5-6 mg of mouse monoclonal IgG or 10-12 mg of polyclonal IgG of most other animal species, per ml of matrix.

APPLICATIONS

  • Purification of antigens by immunoaffinity chromatography.
  • Easy preparation of serum free of antigen.
  • Radio or enzyme immunoassay.

ORDERING INFORMATION

Product code Product name Prepackaged column *Price in EUR
APA-M2 KIT APA-M2 Activated Protein A - Sepharose fast flow column (for APA-M2 KIT) 3x1 ml gel column 230.00
BPA-M2 Binding & Coupling buffer (for APA-M2 KIT) 500 ml
SRP-M2 Saturation reagent (for APA-M2 KIT) 50 ml
* All prices are FOB Liège.
Bulk quantities are available.

INSTRUCTIONS FOR USE WILL BE PROVIDED WITH THE KIT.