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Affiland

Activated PROTEIN A LIKE - SepharoseTM Fast Flow
for especially
IgM, IgY IMMOBILIZATION KIT
(easy-to-use)
For rapid antigen purification by immunoaffinity column

It is not difficult to immobilize a protein on a solid support using any classic activation method such as the cyanogen bromide or N-hydroxysuccinimidylcarboxylate... However, if the protein of interest is a specific immunoglobulin Ig, its biological activity will be affected if not entirely lost after the covalent linkage between the solid support with the terminal H2N of the Ig (IgM, IgY, IgG...).

AFFILAND has developed the Activated Protein A like-Sepharose fast flow useful for the immobilization of Immunoglobulins and particularly IgM, IgY... on the Fc portion, leaving free the Fab: the biological properties of the specific Immunoglobulins will be conserved.

CHARACTERISTICS OF THE GEL

Matrix: SepharoseTM 4 Fast Flow
Ligand: Activated Protein A like which has a specific binding for the Fc of immunoglobulins. It does not recognize serum albumin or serum proteins other than immunoglobulins.
Rapid formation of a stable complex between the ligand and the Fc portion of immunoglobulin from all animal species, in the binding & coupling buffer, followed by an immediate covalent linkage between the Activated matrix and the Fc portion, leaving free the Fab of immunoglobulin.
Easily realized in a column.
Sample: can be either the antisera, ascites fluid, extract containing antibodies or purified immunoglobulins.
Stability of the matrix: pH 6-9.
Storage: 4C in PBS 50mM pH 7.4; NaN3 0.02% (w/v).

Results

Easy recovery of the antibody immobilized on the gel in a record time of 30 minutes maximum. The capacity of the obtained affinity matrix may be 5-6 mg of mouse monoclonal IgG or 10-12 mg of polyclonal IgG of all animal species, per ml of matrix.

APPLICATIONS

  • Purification of antigens by immunoaffinity chromatography.
  • Easy preparation of serum free of antigen.
  • Radio or enzyme immunoassay.

ORDERING INFORMATION

Product code Product name Prepackaged column *Price in EUR
APAL-M1 KIT APAL-M1 Activated Protein A like-Sepharose fast flow column (for APAL-M1 KIT) 3x1 ml gel column 250.00
BPAL-M1 Binding & Coupling buffer (for APAL-M1 KIT) 500 ml
SRPL-M1 Saturation reagent (for APAL-M1 KIT) 50 ml
* All prices are FOB Liège.
Bulk quantities are available.

INSTRUCTIONS FOR USE WILL BE PROVIDED WITH THE KIT.