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IMAC Purification
How to remove 'heavy metal ions' from your protein solution after IMAC elution steps?
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HISTIDINE CONTAINING
PROTEIN PURIFICATION KIT
or PDC KIT
(pentadentate chelator kit)
Ready and easy-to-use

For rapid purification of 6x His-tagged proteins in a single step.
For rapid purification of Zn proteins in a single step.
For first attempts at general purification of proteins.

For more information, see PDC & PDC KIT.

using
  • PDC metal chelate columns, ready-to-use
    • Matrix : SepharoseTM CL, SepharoseTM Fast Flow.
    • Chelated metal ions : Cu2+, Ni2+, Zn2+ & Co2+.
    • Binding capacity: 15-20 mg of 6x His-tagged HSP 60/ml of wet Zn-PDC resin and approx. 15 mg of human alpha2-Macroglobulin/ml of wet Ni-PDC resin.
    • Stability of the resin: 4C-30C and pH 2-11.
    • The gel can be regenerated: minimum 10 cycles without significant loss of binding capacity.
  • And four buffers, ready-to-use
    • Buffer A, B.
    • Buffer E, F.

  Ordering information: PDC

  PDC (Pentadentate chelator) for IMAC (Immobilized metal ion affinity chromatography)