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p-AMINOBENZAMIDINE-MATRIX

CHARACTERISTICS OF THE GEL

Matrix: SepharoseTM CL-4B
and/or SepharoseTM 4 Fast Flow
Activation method: Oxiran.
Binding capacity: Approx. 10 mg of trypsin per ml of wet matrix.
Package: Suspension in PBS 50mM pH 7.4; NaN3 0.02% (w/v).
Shelf life of the matrix: 12 months after delivery when stored at 4C.
Storage: 4C.

ORDERING INFORMATION

Product code Product name Package *Price in EUR
BEN46-4S p-Aminobenzamidine SepharoseTM
CL-4B
Matrix
25 ml 160.00
50 ml 265.00
100 ml 475.00
BEN46-4FF p-Aminobenzamidine SepharoseTM
4 Fast Flow
Matrix
25 ml 205.00
50 ml 350.00
100 ml 620.00
* All prices are FOB Liège.
Bulk quantities are available.

INSTRUCTION FOR USE (Trypsin purification)

By kind personal communication of Dr DEBY-DUPONT, Laboratory of Biochemistry and Radiobiology - University of Lige (Belgium).

Important note: All operations were carried out at 0C-4C.
Gel volume: 25 ml p-Aminobenzamidine-Agarose.
Sample: Extract of human or sheep pancreas, dialysed against the buffer A (see below).
Flow rate: 30 ml/cm2/hour for loading and washing. 50 ml/cm2/hour for elution.
Binding and washing buffer: Tris.HCl 0.1M, NaCl 0.5M pH 8.0 (buffer A).
Elution buffers: 1. AcONa 0.1M, NaCl 0.5M pH 4.0. / 2. Glycine.HCl 0.1M, NaCl 0.05M pH 2.0-2.5.

ABSORBANCE