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®PMIB
(Polyvalent metal ion binding resin)
Easy-to-use
References:
Cyanogen Chloride Precursor
Analysis in Chlorinated River Water
Jun Hee Lee et al; Environmental and Water Resource Engineering, University of Michigan, Ann Arbor, Michigan 48109-2115
Environ. Sci. Technol. 2006, 40, 1478-1484
Note that IDA resins (Iminodiacetic acid resins) are not able to retain the metal ions demanding the octahedral configuration as the stable complex form such as Ca2+, Fe2+ or Fe3+, Ni2+, Co2+...
The PATENTED polyvalent metal ion resin (PMIB) developed by AFFILAND® is capable of forming stable octahedral complexes with all heavy metal ions at the physiological pH.
CHARACTERISTICS OF THE RESIN
Matrix: Approx. 100 µm (dry bead size).
Ligand: Pentadentate chelator.
Flow rate: Very high.
High binding capacity for polyvalent metal ions: approx. 150 µmoles of metal ions per ml of resin. The chelation complexes are stable.
No detectable binding for monovalent metal ions (Na+, K+...).
No detectable binding for proteins.
Long life & regenerable resin: Minimum 20 cycles without significant loss of binding capacity with
regeneration for routine use.
METAL ION BINDING CAPACITY OF PMIB
| Binding capacity/ml resin | ||||
|---|---|---|---|---|
| Metal ion | Formula | M.W. | in mg | in mmoles |
| Ca2+ | CaCl2.2H2O | 147.00 | 26.7 | 0.18 |
| Cu2+ | CuSO4.5H20 | 249.68 | 34.7 | 0.14 |
| Co2+ | CoCl2.6H2O | 237.93 | 34.8 | 0.15 |
| Ba2+ | BaCl2.2H2O | 244.28 | 33.3 | 0.14 |
| Fe3+ | Fe2(SO4)3.nH2O | 399.88 | 30.0 | 0.08 |
| Hg2+ | HgCl2 | 271.5 | 46.7 | 0.17 |
| Mn2+ | MnCl2.4H2O | 197.91 | 29.3 | 0.15 |
| Ni2+ | NiCl2.6H2O | 237.69 | 34.7 | 0.15 |
| Pb2+ | Pb(AcO)2.3H2O | 379.34 | 56.7 | 0.15 |
| Zn2+ | ZnCl2 | 136.30 | 24.0 | 0.18 |
APPLICATIONS
EASY , RAPID & ECONOMIC PREPARATION OF
- Water free of polyvalent metal ions.
- Buffers free of polyvalent metal ions.
- Proteins free of polyvalent metal ions after IMAC purification. In IMAC, the leaching of metal ions due to the stability of the complex Metal-Chelator, can be observed during the equilibration of the column as well as during the chromatography comprising the sample loading, the washing and the elution steps.
- Metalloproteins free of polyvalent metal ions.
ORDERING INFORMATION
* All prices are FOB Liège.
Product code
Product name
Package
*Price in EUR
PMIB
Polyvalent metal ion binding resin
25 ml
250.00
100 ml
700.00
500 ml
2500.00
From 1000 ml
Please inquire
Bulk quantities are available.
The INSTRUCTION FOR USE is provided with the products.
Iminodiacetic acid-Agarose for IMAC PDC (pentadentate chelator) & PDC kit for IMAC