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Ready and Easy-to-use

PDC
(Pentadentate chelator) and
PDC KIT
(Pentadentate chelator kit)

References and applications:
  1. J. Biol. Chem., 275: 11147-11153 (2000) ; 275: 25411-25417 (2000) ; 277: 2413-2418 (2002) ; 278: 10697-10706 (Mar 2003) ; 278, Issue 21: 18817-18823 (May 23, 2003) ; 278: 30677-30685 (Aug 2003).
  2. Eur. J. Biochem., 268: 1613-1619 (2001) ; 269: 3531-3539 (2002) ; 269: 4789-4798 (2002).
  3. Protein Sci., 11: 500-515 (2002) ; 11: 1506-1518 (2002).
  4. Biochem. J., 352: 651-658 (2000).
  5. J. Bacteriol., Oct 2003 ; 185: 5925-5935.
  6. PLANT MOLECULAR BIOLOGY ; Volume 56, Number 6 / December, 2004
  7. Molecular Cell 21, 775-785, March 17, 2006 (C)2006 Elsevier Inc. DOI 10.1016/j.molcel.2006.01.033
  8. Journal of Immunological Methods, Volume 320, Issues 1-2, 30 March 2007, Pages 81-93.
  9. PNAS | November 13, 2007 | vol. 104 | no. 46 | 18157-18162.

  • for 6x His-tagged protein purification.
    and/or
  • for Zn-protein purification in a single step.
    and/or
  • for first attempts at general purification of proteins.


NOW AVAILABLE:
PDC SepharoseTM CL-4B Matrix
PDC SepharoseTM 4 Fast Flow Matrix
PDC SepharoseTM 6 Fast Flow Matrix New!
PDC StreamLine® Quartz Base Matrix New!

The patented pentadentate chelator coupled to SepharoseTM CL-4B (PDC-Agarose) developed by AFFILAND, is capable of forming complexes with all polyvalent metal ions including Cu2+, Ni2+, Zn2+, and Co2+, to give

  • an octahedral Metal ion-Chelator complex.
  • with five coordination sites occupied by the chelator.
This results
  1. in the best stability of the Metal ion-Chelator complex. No leakage of Ni2+, Zn2+ and Co2+ was observed during chromatography steps, including steps using chaotropic buffers such as guanidine hydrochloride 6M pH 8.0, urea 8M pH 8.0 or non-ionic detergents... Note that the guanidine hydrochloride 4M-6M tends to strip slowly the Cu-Chelator column if excessively used.
  2. in one and only one site free for interaction and selective binding of accessible cysteine/histidine residues and chiefly histidine containing biomolecules.
In addition, PDC-Agarose has a high binding capacity:
  • 35-40 µmoles of M2+ (M = Cu, Ni, Zn, Co) per milliliter of wet gel.
  • 15-20 mg of 6x His-tagged HSP 60/ml of wet Zn-PDC gel.
  • approx. 15 mg of human alpha2-Macroglobulin/ml of wet Ni-PDC gel.
    and approx. 15 mg of Mutated triosephosphate isomerase/ml of wet Ni-PDC gel.
  • Stability of the gel:
    • pH range 2-12.
    • Several years at physiological pH if stored at 4°C.
  • The gel can be regenerated : minimum 10 cycles without significant loss of binding capacity.

SepharoseTM: trade mark of GE Healthcare companies.

  6x His-tagged protein purification with PDC using PDC KIT
  Zinc protein purification with PDC using PDC KIT
  General purification of proteins with PDC using PDC KIT
  Ordering information
  Iminodiacetic acid-Agarose for IMAC
  PMIB (polyvalent metal ion binding resin) for water, buffers & proteins free of polyvalent metal ions