Nitration of tyrosine residues occurs in various cell types and depends mainly, in vivo or in vitro, on the formation of peroxynitrite from singulet oxygen and nitric oxyde.

Nitration of tyrosine residues in proteins could interfere with many transduction pathways since nitration of tyrosine residues prevents phosphorylation of this amino-acid and therefore its recognition by specific motifs.

Nitration is so one of causes of physiological process like inflammation, immune response and atherosclerosis.

Matrix: Sepharose^TM 4 Fast Flow.
Spacer arm: 12 atoms.
Ligand: Rabbit IgG anti-nitrotyrosine containing peptides.
Attachment: at Fc portion of IgG.
Immobilized ligand: 10,000 µg of Rb IgG per ml of resin.
Affinity resin volume: 400 µl wet resin.
Specificity: determined by RIA - 4 µg of nitrotyrosine containing peptides per 400 µl of resin column.
Storage: 4°C, suspension in PBS buffer, pH 7.4, in the presence of NaN₃ 0.1% (w/v).

ORDERING INFORMATION

Product code	Product name	Quantity	*Price in EUR
Rb IgG-NT-c	Rabbit IgG anti-nitrotyrosine containing peptide column	1 ea	365.00
		5 ea	1734.00
		> 10 ea	Please inquire
Rb IgG-NT-a	Rabbit IgG anti-nitrotyrosine containing peptide antibody	1000 µg	149.00

* All prices are FOB Liège.
Bulk quantities are available.

Shipment at room temperature

  Protein Phosphorylation Analysis
  Specific Affinity Resins for Albumin et IgG Depletion