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Affiland

PROTEIN PHOSPHORYLATION ANALYSIS

The human genome encoded 518 protein kinases and 65 protein phosphatases which control reversible phosphorylation of proteins.
However, only about 0.05% of protein phosphorylation occurs on Tyrosine residues in mammals. Thus profiling Tyrosyl phosphorylation appears very challenging without enrichment of phosphoproteins or phosphopeptides.
Immunoprecipitation, immobilized metal affinity chromatography, methylation of peptides, nanoflow reversed phase were combined with mass spectroscopy to detect theses modified peptides in the mixture.
Affiland has developed a set of powerful Metal Chelate Affinity Chromatography to enrich the phosphopeptides.

  • Enrichment of phosphotyrosine containing proteins

by

New! Rb IgG anti-phosphotyrosine New!
Immunoaffinity Column

Matrix: SepharoseTM 4 Fast Flow.
Spacer arm: 12 atoms.
Ligand: Rabbit IgG anti-phosphotyrosine.
Attachment: at Fc portion of IgG.
Immobilized ligand: 10,000 g of Rb IgG per ml of resin.
Affinity resin volume: 400 l wet resin.
Specificity: Binding capacity determined by RIA : 6 g of phosphtyrosine containing proteins per 400 l of resin column.
Storage: 4C, suspension in PBS buffer, pH 7.4, in the presence of NaN3 0.1% (w/v).

ORDERING INFORMATION

Product code Product name Quantity *Price in EUR
Rb IgG-PhosT-c Rabbit IgG anti-phosphotyrosine column 1 ea 365.00
5 ea 1734.00
> 10 ea Please inquire
Rb IgG-PhosT-a Rabbit IgG anti-phosphotyrosine antibody 1000 g 149.00
* All prices are FOB Liège.
Bulk quantities are available.

Shipment at room temperature

 

  • Enrichment of phosphotyrosine containing proteins

by

Metal chelate Affinity Chromatography
our powerful patented Pentadentate chelator

Matrix: SepharoseTM 4 Fast Flow.
Spacer arm: 12 atoms.
Ligand: Pentadentate chelator®.
For details, please see http://www.affiland.com/imac/pdc.htm
Storage: 4C, suspension in PBS buffer, pH 7.4, in the presence of NaN3 0.1% (w/v).
®: patented Affiland product.

ORDERING INFORMATION

Product code Product name Quantity *Price in EUR
Fe-PDC-4FF-c
and/or
Gn-PDC-4FF-c
Fe(III) Pentadentate chelator-Sepharose 4 fast flow, 1ml resin column
and/or
Gn(III) Pentadentate chelator-Sepharose 4 fast flow, 1ml resin column
5 ea 174.00
10 ea 271.00
> 10 ea Please inquire
* All prices are FOB Liège.
Bulk quantities are available.

Shipment at room temperature


  Detection of Nitro-Tyrosine
  Specific Affinity Resins for Albumin et IgG Depletion